Composite

Part:BBa_K4743003

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-09-16)


NadB+ADH1 terminator with adapter

It codes for L-aspartate oxidase, which is an enzyme that involved in NAD synthesis.It turns aspartate into Iminoaspartate. It is involved in the pathway: NAD biosynthesis, aspartate => quinolinate => NAD

Length checking of nadB

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadB.

Overall metabolic pathway of NMN

NadB is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 2. The Matabolic pathway of NMN production.

References

1.Seifert, J., Kunz, N., Flachmann, R., Läufer, A., Jany, K. D., & Gassen, H. G. (1990). Expression of the E. coli nadB gene and characterization of the gene product L-aspartate oxidase. Biological chemistry Hoppe-Seyler, 371(3), 239–248.

2.Cookson, B. T., Olivera, B. M., & Roth, J. R. (1987). Genetic characterization and regulation of the nadB locus of Salmonella typhimurium. Journal of bacteriology, 169(9), 4285–4293. https://doi.org/10.1128/jb.169.9.4285-4293.1987

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1833
    Illegal XbaI site found at 702
    Illegal XbaI site found at 921
    Illegal XbaI site found at 1167
    Illegal XbaI site found at 1264
    Illegal SpeI site found at 1638
    Illegal PstI site found at 1034
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1833
    Illegal SpeI site found at 1638
    Illegal PstI site found at 1034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1833
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1833
    Illegal XbaI site found at 702
    Illegal XbaI site found at 921
    Illegal XbaI site found at 1167
    Illegal XbaI site found at 1264
    Illegal SpeI site found at 1638
    Illegal PstI site found at 1034
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1833
    Illegal XbaI site found at 702
    Illegal XbaI site found at 921
    Illegal XbaI site found at 1167
    Illegal XbaI site found at 1264
    Illegal SpeI site found at 1638
    Illegal PstI site found at 1034
    Illegal AgeI site found at 1846
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 801


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